Ornithinimicrobium pratense sp. nov., isolated from meadow soil. Issue 12 (11th December 2020)

• Record Type: Journal Article
• Authors: Guo, Qiannan; Wang, Bo; Zhou, Zhengfu; Ke, Xiubin; Zhang, Lijuan; Chen, Ming; Lin, Min; Wang, Wei; Zhang, Wei; Li, Xufeng
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 12(2020)
• Page Start: 6450
• Page End: 6457
• Publication Date: 2020-12-11
• Subjects: meadow soil -- Ornithinimicrobium -- polyphasic approach -- whole-genome sequencing; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004557
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204 T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204 T was closely related to Ornithinimicrobium flavum CPCC 203535 T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582 T (96.9 %), Serinicoccus sediminis GP-T3-3 T (96.8 %), Serinicoccus hydrothermalis JLT9 T (96.7 %), Ornithinimicrobium cerasi CPCC 203383 T (96.6 %) and Ornithinimicrobium kibberense K22-20 T (96.6 %). However, the digital DNA–DNA genome hybridization value between strain W204 T and the closest related strain O. flavum CPCC 203535 T was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H4 ). The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0 . The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204 T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp. nov. is proposed. The type strain is W204 T (=GDMCC 1.1391 T =KCTC 49237 T ).