Steptomyces fagopyri sp. nov., a novel actinomycete isolated from rhizospheric soil of Fagopyrum dibotrys. Issue 12 (12th December 2020)

• Record Type: Journal Article
• Authors: Guo, Yihui; Tang, Xinke; Hu, Siren; Li, Kaiqin; Zhou, Meiliang; Jian, Gao
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 12(2020)
• Page Start: 6437
• Page End: 6443
• Publication Date: 2020-12-12
• Subjects: polyphasic taxonomy -- rhizosphere soil -- Streptomyces fagopyri sp. nov.; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004555
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel actinomycete, designated strain QMT-28 T, was isolated from rhizosphere soil of Fagopyrum dibotrys collected from Shuangfeng, Hunan Province, PR China. Strain QMT-28 T grew well on International Streptomyces Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into loose spiral spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was ll -diaminopimelic acid and the whole-cell sugars were galactose and glucose. The predominant fatty acids were C18 : 1 cis 9, summed feature 6 (C18 : 2 cis 9, 12/C18 : 0 a) and C16 : 0 . The polar lipids included diphosphatidylglycerol, hydroxy phospatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, phospholipids of unknown structure containing glucosamine and several unidentified phospholipids. The major menaquinones were MK-9, MK-9(H2 ), MK-9(H4 ), MK-9(H6 ) and MK-9(H8 ). The genome size of strain QMT-28 T was about 8.7 Mbp with a G+C content of 71.2 mol%. Phylogenetic analysis showed that the novel strain was closely related to Streptomyces olivochromogenes DSM 40451 T (99.5 % similarity), Streptomyces mirabilis NBRC 13450 T (98.9 %), Streptomyces kanamyceticus NBRC 13414 T (98.9 %), Streptomyces kaempferi I37 T (98.9 %) and Streptomyces arcticus ZLN234 T (98.8 %). However, the average nucleotide identity values, the digital DNA–DNA hybridization values and the multilocus sequence analysis evolutionary distances between this strain and closely related strains showed that it belonged to a distinct species. In addition, these results were also supported by differences in the phenotypic characteristics between QMT-28 T and five closely related type strains. Consequently, strain QMT-28 T should represent a novel species of the genus Streptomyces, with the suggested name Streptomyces fagopyri sp. nov. The type strain is QMT-28 T (=CICC 24808 T =JCM 33796 T ).