Chitinophaga extrema sp. nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest. Issue 12 (4th December 2020)

• Record Type: Journal Article
• Authors: Goh, Calvin Bok Sun; Wong, Li Wen; Parimannan, Sivachandran; Rajandas, Heera; Loke, Stella; Croft, Lawrence; Yule, Catherine Mary; Pasbakhsh, Pooria; Lee, Sui Mae; Tan, Joash Ban Lee
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 12(2020)
• Page Start: 6355
• Page End: 6363
• Publication Date: 2020-12-04
• Subjects: Chitinophaga extrema -- Malaysia -- Phylogeny -- Taxonomy -- Tropical Peat Swamp -- Koompassia malaccensis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004539
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1 T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a Koompassia malaccensis tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39′ 04.7′ N 101° 17′ 43.7′′ E). Phylogenetic analyses based on the full 16S rRNA sequence revealed that strain Mgbs1 T belongs to the genus Chitinophaga with the greatest sequence similarity to Chitinophaga terrae KP01 T (97.65 %), Chitinophaga jiangningensis DSM27406 T (97.58 %), and Chitinophaga dinghuensis DHOC24 T (97.17 %). The major fatty acids of strain Mgbs1 T (>10 %) are iso-C15 : 0, C16 : 1 ω 5 c and iso-C17 : 0 3-OH while the predominant respiratory quinone is menaquinone-7. Strain Mgbs1 T has a complete genome size of 8.03 Mb, with a G+C content of 48.5 mol%. The DNA–DNA hybridization (DDH) score between strain Mgbs1 T and C. jiangningensis DSM27406 T was 15.9 %, while in silico DDH values of strain Mgbs1 T against C. dinghuensis DHOC24 T and C. terrae KP01 T were 20.0 and 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores between strain Mgbs1 T against all three reference strains are 73.2 %. Based on the phenotypic, chemotaxonomic, and phylogenetic consensus, strain Mgbs1 T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga extrema sp. nov. is proposed (=DSM 108835 T =JCM 33276 T ).