An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy. Issue 12 (25th December 2020)

• Record Type: Journal Article
• Authors: Söderström, Bill; Ruda, Alessandro; Widmalm, Göran; Daley, Daniel O.
• Is Part Of: Microbiology. Volume 166:Issue 12(2020)
• Page Start: 1129
• Page End: 1135
• Publication Date: 2020-12-25
• Subjects: FDAA -- FLDA -- OGDA -- OregonGreen488 -- peptidoglycan -- transpeptidase; Microbiology -- Periodicals; 579
• DOI: 10.1099/mic.0.000996
• Languages: English
• ISSNs: 1350-0872

Abstract

Abstract : Fluorescent d -amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an OregonGreen488-labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Gram-positive and some Gram-negative bacteria, and imaged by super-resolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm.