UHPLC–MS/MS method for the determination of azithromycin in human plasma of pediatric patients. (2nd June 2022)

• Record Type: Journal Article
• Authors: Cai, Ningfang; Wu, Xiuping; Lin, Jiancheng; Zou, Jialiang; Yi, Jin Ling; Luo, Xi
• Is Part Of: Biomedical chromatography. Volume 36:Number 9(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-06-02
• Subjects: azithromycin -- interleukin‐6 -- interleukin‐10 -- LC‐MS/MSpolymorphism; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.5415
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: The presence of azithromycin in the human plasma of pediatric patients was determined with a UHPLC‐MS/MS assay. Sample preparation was done by protein precipitation, and the separation was achieved on a C18 column by the gradient mixture of the mobile phase A (0.1% acetic acid and 3 mM ammonium acetate in water) and the mobile phase B (0.1% acetic acid and 3 mM ammonium acetate in the solution of acetonitrile, methanol, and water, 47.5/47.5/5, V/V/V). The multiple reaction monitoring mode was adopted to monitor the precursor‐to‐product ion transitions of m/z 749.6 → m/z 591.5 for azithromycin and m / z 753.6 → m/z 595.5 for azithromycin‐ 13 C‐d3 (the internal standard) at the positive ionization mode. The calibration curve ranged from 0.5 to 500.0 ng·mL −1, and the correlation coefficient was greater than 0.99. The intra‐ and inter‐batch precision was less than 13.7%. Accuracy determined at four concentrations ranged from 99.5 to 110.8%. The extraction recoveries were more than 95%, and the matrix effects were 98–100%. The stability under various conditions was acceptable with the accuracy deviation within 9.2%. In conclusion, our method was simple, sensitive, and reliable for quantifying azithromycin in plasma among pediatric patients.