Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification. Issue 1 (22nd February 2018)

• Record Type: Journal Article
• Authors: Ma, Zexu; Yang, Liping; Zhang, Yan‐Jin
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 48:Issue 1(2018)
• Page Start: 15M.1.1
• Page End: 15M.1.14
• Publication Date: 2018-02-22
• Subjects: infectious clone -- PRRSV -- propagation -- quantification; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.51
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family Arteriviridae, order Nidovirale . PRRSV is an enveloped, single‐stranded, positive‐sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC‐145 cell line is the most often used in a laboratory setting. Infectious cDNA clones of many PRRSV strains have been established, from which these viruses can be recovered. PRRSV titration is generally done in MARC‐145 cells. PRRSV RNA copy numbers can be assessed by reverse transcription and real‐time PCR. Here, protocols for PRRSV propagation, virus recovery from infectious cDNA clones, and quantification are presented. © 2018 by John Wiley & Sons, Inc.