Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study. Issue 1 (14th December 2020)
- Record Type:
- Journal Article
- Title:
- Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study. Issue 1 (14th December 2020)
- Main Title:
- Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study
- Authors:
- Presicce, Giorgio Antonio
Neglia, Gianluca
Salzano, Angela
Padalino, Barbara
Longobardi, Valentina
Vecchio, Domenico
De Carlo, Esterina
Gasparrini, Bianca - Abstract:
- Abstract: The study evaluated the effects of eCG treatment prior to ovum pick up (OPU) on follicular population, oocyte and embryo yields in summer and autumn in Podolic cattle. The effects of repeated OPU on cattle wellbeing was also documented. Twenty-six animals were used, and split into two groups, treatment (OPU; n = 18) and control (CG; n = 8). The OPU cattle were subsequently split into two subgroups ( n = 9) and underwent repeated OPU, without and with eCG priming, for a total of 8 sessions (4 sessions/season). Follicular population, oocyte and embryo yields were recorded in those sub-groups. CG was handled in the same manner of OPU, except for epidural anaesthesia and follicular aspiration. Biochemical profile, serum protein electrophoresis and haptoglobin levels were analysed in OPU and CG. Hormonal priming increased the number of medium follicles (1.7 ± 0.2 vs 0.9 ± 0.2, p < .05), while it decreased the recovery rate and number of cumulus-enclosed oocytes (COCs) (recovery rate: 38.3 ± 3.5 vs 60.5 ± 4.0%; COCs: 2.3 ± 0.3 vs 3.4 ± 0.4, respectively; p < .01). However, priming increased cleavage (72.9 ± 5.7 vs 49.4 ± 5.4; p < .05) and blastocyst (41.1 ± 5.7 vs 23.0 ± 4.2; p = .054) rates. With regard to season's effect, a higher number of COCs was recorded in autumn than in summer (3.1 ± 0.4 vs 2.6 ± 0.3; p < .05) without affecting though the number of embryos produced (0.9 on average). Since haematological parameters did not vary between OPU and CG, ourAbstract: The study evaluated the effects of eCG treatment prior to ovum pick up (OPU) on follicular population, oocyte and embryo yields in summer and autumn in Podolic cattle. The effects of repeated OPU on cattle wellbeing was also documented. Twenty-six animals were used, and split into two groups, treatment (OPU; n = 18) and control (CG; n = 8). The OPU cattle were subsequently split into two subgroups ( n = 9) and underwent repeated OPU, without and with eCG priming, for a total of 8 sessions (4 sessions/season). Follicular population, oocyte and embryo yields were recorded in those sub-groups. CG was handled in the same manner of OPU, except for epidural anaesthesia and follicular aspiration. Biochemical profile, serum protein electrophoresis and haptoglobin levels were analysed in OPU and CG. Hormonal priming increased the number of medium follicles (1.7 ± 0.2 vs 0.9 ± 0.2, p < .05), while it decreased the recovery rate and number of cumulus-enclosed oocytes (COCs) (recovery rate: 38.3 ± 3.5 vs 60.5 ± 4.0%; COCs: 2.3 ± 0.3 vs 3.4 ± 0.4, respectively; p < .01). However, priming increased cleavage (72.9 ± 5.7 vs 49.4 ± 5.4; p < .05) and blastocyst (41.1 ± 5.7 vs 23.0 ± 4.2; p = .054) rates. With regard to season's effect, a higher number of COCs was recorded in autumn than in summer (3.1 ± 0.4 vs 2.6 ± 0.3; p < .05) without affecting though the number of embryos produced (0.9 on average). Since haematological parameters did not vary between OPU and CG, our preliminary data suggest that repeated OPU may be used as a conservation strategy in Podolic cattle without affecting wellbeing. Highlights: Podolic cattle is an endangered Italian breed, reared in semi-extensive/extensive systems Ovum pick-up (OPU) can be carried out in this breed without impairing animal health and welfare The eCG treatment before OPU (priming) did not improve the number of embryos produced per donor. … (more)
- Is Part Of:
- Italian journal of animal science. Volume 19:Issue 1(2020)
- Journal:
- Italian journal of animal science
- Issue:
- Volume 19:Issue 1(2020)
- Issue Display:
- Volume 19, Issue 1 (2020)
- Year:
- 2020
- Volume:
- 19
- Issue:
- 1
- Issue Sort Value:
- 2020-0019-0001-0000
- Page Start:
- 31
- Page End:
- 40
- Publication Date:
- 2020-12-14
- Subjects:
- Conservation strategy -- Podolic cattle -- embryo production -- wellbeing
Animal culture -- Periodicals
Livestock -- Italy -- Periodicals
Veterinary medicine -- Italy -- Periodicals
Animal culture
Livestock
Veterinary medicine
Italy
Periodicals
Periodicals
636.005 - Journal URLs:
- http://bibpurl.oclc.org/web/23047 http://www.aspajournal.it/default.htm ↗
http://search.ebscohost.com/direct.asp?db=a9h&jid=783N&scope=site ↗
http://www.aspajournal.it/ ↗
http://www.aspajournal.it/index.php/ijas ↗
http://www.tandfonline.com/loi/tjas20 ↗
http://www.tandfonline.com/loi/tjas20/current ↗
http://www.tandfonline.com/ ↗ - DOI:
- 10.1080/1828051X.2019.1684213 ↗
- Languages:
- English
- ISSNs:
- 1828-051X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 23833.xml