ELISA for Molluscum Contagiosum Virus. Issue 1 (9th November 2017)

• Record Type: Journal Article
• Authors: Sherwani, Subuhi; Chowdhury, Mohammed; Bugert, Joachim J.
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 47:Issue 1(2017)
• Page Start: 14A.6.1
• Page End: 14A.6.9
• Publication Date: 2017-11-09
• Subjects: E. coli BL21 RIl+ expression system -- molluscum contagiosum virus -- GST fusion proteins -- MC084 -- MC133 -- H3L -- ELISA; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.42
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Molluscum contagiosum virus (MCV) is a common skin pathogen of children and young adults. Infection with MCV causes benign skin tumors in children and young adults and is mostly self‐limiting. In contrast to orthopoxviruses, MCV infections tend to take a subacute clinical course but may persist for up to 12 months. Current numbers for MCV seroprevalence in different geographical areas are based on a variety of historical serological methods from complement fixation assays to MCV ELISAs based on purified MCV virions and MC133 antigen expressed in a Semliki Forest Virus expression system. A standardized ELISA for the assessment of MCV seroprevalence would be useful to determine global MCV seroprevalence. The methods described show that polypeptides derived from MCV open reading frames MC084 (residues V123 to R230 and V33 to G117), mc133 (residues M1 to N370), and glutathione S‐transferase (GST)‐H3L (residues I142 to W251) expressed in E. coli RIL+ as GST fusion proteins can be used to assess antibody binding in a GST capture ELISA. We show how the ELISA can be used to screen a panel of patient sera previously characterized with the mc084 V123‐R230 ELISA. © 2017 by John Wiley & Sons, Inc.