The effect of combined pulsed wave low‐level laser therapy and mesenchymal stem cell‐conditioned medium on the healing of an infected wound with methicillin‐resistant Staphylococcal aureus in diabetic rats. Issue 7 (25th March 2018)

• Record Type: Journal Article
• Authors: Kouhkheil, Reza; Fridoni, Mohammadjavad; Piryaei, Abbas; Taheri, Soudabeh; Chirani, Alireza S.; Anarkooli, Iraj J.; Nejatbakhsh, Reza; Shafikhani, Sasha; Schuger, Lucia A.; Reddy, Vijaya B.; Ghoreishi, Seyed Kamran; Jalalifirouzkouhi, Reza; Chien, Sufan; Bayat, Mohammad
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 7(2018)
• Page Start: 5788
• Page End: 5797
• Publication Date: 2018-03-25
• Subjects: diabetes mellitus -- diabetic foot ulcers -- low‐level laser therapy -- human bone marrow mesenchymal stem cell‐conditioned medium -- methicillin‐resistant staphylococcal aureus -- microbial flora; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26759
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: This study aims to investigate the combined effects of Pulsed wave low‐level laser therapy (PW LLLT) and human bone marrow mesenchymal stem cell‐conditioned medium (hBM‐MSC‐CM) on the microbial flora and tensiometrical properties of an infected wound model with methicillin‐resistant staphylococcal aureus (MRSA) in an experimental model for Type 1 diabetes mellitus (TIDM). TIDM was induced in rats by streptozotocin (STZ). One full‐thickness excision was made on the backs of the rats. Next, the rats were divided into the following groups: Group 1 was the control (placebo) group; Group 2 received hBM‐MSCs‐CM four times; Group 3 were laser PWLLLT (890 nm, 80 Hz, 0.2 J/cm 2 ); and Group 4 received hBM‐MSCs‐CM +LASER. Wounds were infected with MRSA. Microbiological examinations were performed on days 4, 7, and 15. Tensiometerical examinations were carried out on the 15th day. One‐way analysis of variance showed that laser and CM alone and/or in combination significantly increases the tensiomerical properties of the repaired wounds compared with control wounds. A combination of PW laser and CM was statistically more effective than other treated groups. Two‐way analysis of variance showed that laser and CM alone and/or in combination significantly decreases the colony‐forming units (CFUs) compared with the control group. The application of hBM‐MSC‐CM and PWlaser alone and/or together significantly accelerates the wound‐healing process in MRSA‐infected cutaneous wounds in TI DM in rats. Additionally, a combined application of hBM‐MSC‐CM and PWlaser demonstrates a synergistic effect on the wound‐healing process in MRSA‐infected cutaneous wounds in Type I DM rats. Abstract : The application of human bone marrow mesenchymal stem cell‐conditioned medium and Pulsed wave low level laser therapy alone and/or together significantly accelerates the wound‐healing process in Methicillin‐Resistant Staphylococcal Aureus‐infected cutaneous wounds in type one diabetic in rats.