Oxidative imbalance and muscular alterations in diverticular disease. Issue 9 (September 2022)

• Record Type: Journal Article
• Authors: Pallotta, Lucia; Vona, Rosa; Maselli, Maria Antonietta; Cicenia, Alessia; Bella, Antonino; Ignazzi, Antonia; Carabotti, Marilia; Cappelletti, Martina; Gioia, Alessia; Tarallo, Mariarita; Tellan, Guglielmo; Fiori, Enrico; Pezzolla, Francesco; Matarrese, Paola; Severi, Carola
• Is Part Of: Digestive and liver disease. Volume 54:Issue 9(2022)
• Page Start: 1186
• Page End: 1194
• Publication Date: 2022-09
• Subjects: Antioxidants -- Diverticulitis -- Human colon -- Smooth muscle; cGMP cyclic guanosine monophosphate -- CGRP neuronal calcitonin gene-related peptide -- COL1A1 collagen 1 -- Ct Threshold Cycle -- DNP 2, 4-dinitrophenylhydrazine -- GAPDH Glyceraldehyde-3-phosphate dehydrogenase -- GDNF glial cell line-derived neurotrophic factor -- GI gastrointestinal -- HMGB1 high-mobility group box protein 1 -- HRP horseradish peroxidase -- MYH11 myosin heavy chain 11 -- NLRP3 NLR family pyrin domain containing 3/ NAcht Leucine-rich repeat Protein 3 -- NRF2 Nuclear Factor Erythroid 2-related Factor 2 -- PDGF-BB Platelet-derived growth factor-BB -- RAGE receptor for advanced glycation end-products -- RQ relative quantification -- SMAD mothers against decapentaplegic homolog -- SMC smooth muscle cells -- SOD superoxide dismutase -- TGF-β Transforming growth factor-beta -- TLRs Toll-Like Receptors -- TNF-α tumor necrosis factor -- TRIB 3 Tribbles-like protein-3 -- VIP Vasoactive intestinal peptide -- α-SMA alpha-smooth muscle actin; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; 616.33005
• DOI: 10.1016/j.dld.2022.02.001
• Languages: English
• ISSNs: 1590-8658

Abstract

Abstract: Background: It is still a matter of debate if neuromuscular alterations reflect a primary event in diverticular disease (DD). Aims: This study aimed to assess colonic wall layers from both stenotic and non-stenotic complicated DD, bio-phenotypic alterations, inflammatory and oxidative status. Methods: A systematic analysis of colonic specimens obtained from stenotic and non-stenotic DD specimens was conducted and compared with controls. Biological activity and qPCR analysis were performed on longitudinal and circular muscles. Western blot analysis was performed throughout colonic wall layers to quantify oxidative and inflammatory markers. Results: A homogenous increase in oxidative stress was observed through all the layers, which were more sharpened in the longitudinal muscle for a loss in antioxidant defenses. In both stenotic and non-stenotic colon, the longitudinal muscle presented an impaired relaxation and a cellular phenotypic switch driven by transforming growth factor-β with an increase in mRNA expression of collagen Iα and a decrease in myosin heavy chain. The circular muscle, as the mucosa, was less affected by molecular alterations. No peculiar increase in inflammatory markers was observed. Conclusion: A longitudinal colonic myopathy is present in DD, independently from the disease stage associated with an oxidative imbalance that could suggest new therapeutic strategies.