Direct PCR of Intact Bacteria (Colony PCR). Issue 1 (13th February 2018)

• Record Type: Journal Article
• Authors: Woodman, Michael E.; Savage, Christina R.; Arnold, William K.; Stevenson, Brian
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 42:Issue 1(2016)
• Page Start: A.3D.1
• Page End: A.3D.7
• Publication Date: 2018-02-13
• Subjects: colony PCR -- bacteria -- screen; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.14
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.