A versatile ceramic capillary membrane reactor system for continuous enzyme‐catalyzed hydrolysis. Issue 8 (9th July 2021)

• Record Type: Journal Article
• Authors: Messner, Lorn; Antink, Marieke H.; Guo, Tongwei; Maas, Michael; Beutel, Sascha
• Is Part Of: Engineering in life sciences. Volume 21:Issue 8/9(2021)
• Page Start: 527
• Page End: 538
• Publication Date: 2021-07-09
• Subjects: antibody fragments -- bioactive peptides -- food proteins -- immobilized enzymes -- proteolysis; Bioengineering -- Periodicals; 660.605
• DOI: 10.1002/elsc.202100027
• Languages: English
• ISSNs: 1618-0240

Abstract

Abstract: As an alternative to classical batch processes, enzyme‐catalyzed hydrolysis can also be carried out continuously. To facilitate this, a continuous ceramic capillary membrane reactor system (CCCMRS) was developed which can be operated with various proteolytic enzymes immobilized on the porous ceramic capillary membranes. This system has several advantages over common batch processes regarding stability, reproducibility and controllability and can easily be adapted to optimal reaction conditions and individual preferences. Two exemplary applications utilizing the CCCMRS were carried out and investigated in long‐term stability studies. In the first application the continuous enzymatic cleavage of human IgG into the antibody fragments Fab and Fc by immobilized papain was performed. A total volume of 22 mL of 1 mg mL ‐1 IgG‐solution was enzymatically cleaved over a period of 33.3 h. The antibody cleavage products could be detected in an SEC‐HPLC over the whole process time thus indicating long‐term stability of the continuous hydrolysis process. The second application investigated the continuous digestion of pea and almond protein isolates by immobilized Alcalase resulting in the generation of a large variety of different peptides. This peptide fingerprint remains constant over a long period of time enabling fractionation and thus making the peptides accessible for further bioactivity studies in sufficient quantities. The constant peptide fingerprint could be shown in the RP‐HPLC analysis for all 30 samples with a total volume of 29.7 mL collected over a period of 45 h.