Cytotoxic and immunomodulator potential of hederagenin saponins from Cephalaria tchihatchewii. (August 2021)

• Record Type: Journal Article
• Authors: Sarikahya, Nazli Boke; Mkouboi, Mohamed Chanfiou; Nalbantsoy, Ayse; Elibol, Murat
• Is Part Of: Phytochemistry letters. Volume 44(2021)
• Page Start: 216
• Page End: 221
• Publication Date: 2021-08
• Subjects: A549 human alveolar adenocarcinoma -- BuOH butanol -- CC column chromatography -- CCD 34Lu normal lung fibroblast cells -- CRL5807 bronchioalveolar carcinoma, non-small cell lung cancer -- CRL 5826 squamous cell carcinoma, mesothelioma -- HeLa human cervix adenocarcinoma -- HTB-177 carcinoma, large cell lung cancer -- MeOH methanol -- MTT [3-(4, 5-dimethyl-2-thiazolyl-2, 5-diphenyl-2H-tetrazolium bromide)]; Caprifoliaceae -- Cephalaria tchihatchewii -- Saponin -- Cytotoxicity -- Immunomodulatory -- 2D NMR analysis; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2020.12.007
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Two new saponins, tchihatchewosides A–B were isolated from C. tchihatchewii. All isolated compounds were identified by extensive spectroscopic techniques. Compound 1a, obtained after alkaline hydrolysis of compound 1 was exhibited moderate cytotoxicity. Tchihatchewosides A–B and prosapogenins 1a-2a stimulated IL1β cytokine release. Abstract: Two newly described oleanane-type saponins (1–2 ), named tchihatchewosides A–B, along with thirteen known compounds, were isolated from the aerial parts of Cephalaria tchihatchewii using several type of chromatography processes. The structures of all compounds (1-15 ) were determined by spectroscopic (1D- and 2D-NMR, HR-ESI/MS) and chemical methods. The cytotoxic activities on CCD 34Lu, A549, CRL5807, CRL 5826, HTB-177 and HeLa cell lines of newly described compounds 1–2 and 1a-2a were evaluated by MTT method using doxorubicin as positive control. Immunomodulatory activity was performed with PMA plus ionomycin in stimulated whole blood cells treated with saponins. The supernatant was analyzed for IL-4, IFN-γ and IL1β cytokines by ELISA. DMSO was considered as the negative control. The results showed that the only compound 1a was exhibited moderate cytotoxicity against CRL 5826 and CCD 34Lu cells. The compounds 1–2 and prosapogenins 1a-2a stimulated IL1β cytokine release, indicating that they might potentially stimulate the innate immune response.