Imbalance of intestinal microbial homeostasis caused by acetamiprid is detrimental to resistance to pathogenic bacteria in Bombyx mori. (15th November 2021)

• Record Type: Journal Article
• Authors: Li, Fanchi; Li, Mengxue; Zhu, Qingyu; Mao, Tingting; Dai, Minli; Ye, Wentao; Bian, Dandan; Su, Wujie; Feng, Piao; Ren, Yuying; Sun, Haina; Wei, Jing; Li, Bing
• Is Part Of: Environmental pollution. Volume 289(2021)
• Publication Date: 2021-11-15
• Subjects: Bombyx mori -- Acetamiprid -- Gut microbiota -- Microbial homeostasis -- Pathogenic infection; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.117866
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The neonicotinoid insecticide acetamiprid is widely applied for pest control in agriculture production, and its exposure often results in adverse effects on a non-target insect, Bombyx mori . However, only few studies have investigated the effects of exposure to sublethal doses of neonicotinoid insecticides on gut microbiota and susceptibility to pathogenic bacteria. In this study, we aimed to explore the possible mechanisms underlying the acetamiprid-induced compositional changes in gut microbiota of silkworm and reduced host resistance against detrimental microbes. This study indicated that sublethal dose of acetamiprid activated the dual oxidase-reactive oxygen species (Duox-ROS) system and induced ROS accumulation, leading to dysregulation of intestinal immune signaling pathways. The evenness and structure of bacterial community were altered. Moreover, after 96 h of exposure to sublethal dose of acetamiprid, several bacteria, such as Pseudomonas sp (Biotype A, DOP-1a, XW34) and Staphylococcus sp (RCB1054, RCB314, X302), invaded the silkworm hemolymph. The survival rate and bodyweight of the acetamiprid treated silkworm larvae inoculated with Enterobacter cloacae ( E. cloacae ) were significantly lower than the acetamiprid treatment group, suggesting that acetamiprid reduced silkworm resistance against pathogens. These findings indicated that acetamiprid disturbed gut microbial homeostasis of Bombyx mori, resulting in changes in gut microbial community and susceptibility to detrimental microbes. Graphical abstract: Image 1 Highlights: Sublethal doses of acetamiprid activated the Duox-ROS system and induced ROS production. Sublethal doses of acetamiprid dysregulated intestinal IMD immune signaling pathways. Acetamiprid perturbs intestinal microbial homeostasis and changed microbial community. Acetamiprid reduces silkworm resistance against harmful microbes.