A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures. Issue 1 (1st July 2013)

• Record Type: Journal Article
• Authors: Dolman, Nick J.; Kilgore, Jason A.; Davidson, Michael W.
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dobrucki, Jurek; Hyun, William C.; Nolan, John P.; Orfao, Alberto; Rabinovitch, Peter S.
• Is Part Of: Current protocols in cytometry. Volume 65:Issue 1(2013)
• Page Start: 12.30.1
• Page End: 12.30.27
• Publication Date: 2013-07-01
• Subjects: vesicles -- autophagosomes -- endosomes -- peroxisomes -- lysosomes -- labeling -- imaging -- fluorescent dyes -- fluorescent proteins -- BacMam; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy1230s65
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of BacMam constructs has allowed more easy‐to‐use choices. Presented here is a discussion of BacMam constructs as well as a review of commercially‐available reagents for labeling vesicular structures in cells, including endosomes, peroxisomes, lysosomes, and autophagosomes, complete with a featured reagent for each structure, recommended protocol, troubleshooting guide, and example image. Curr. Protoc. Cytom . 65:12.30.1‐12.30.27. © 2013 by John Wiley & Sons, Inc.