Cyanine‐Dyad Molecular Probe for the Simultaneous Profiling of the Evolution of Multiple Radical Species During Bacterial Infections. (28th June 2021)

• Record Type: Journal Article
• Authors: Wang, Zhimin; Cong, Thang Do; Zhong, Wenbin; Lau, Jun Wei; Kwek, Germain; Chan‐Park, Mary B.; Xing, Bengang
• Is Part Of: Angewandte Chemie. Volume 133:Number 31(2021)
• Page Start: 17037
• Page End: 17042
• Publication Date: 2021-06-28
• Subjects: bacterial infections -- multiple radical dynamics -- NIR fluorescence imaging -- small-molecule probes; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202104100
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Real‐time monitoring of the evolution of bacterial infection‐associated multiple radical species is critical to accurately profile the pathogenesis and host‐defense mechanisms. Here, we present a unique dual wavelength near‐infrared (NIR) cyanine‐dyad molecular probe (HCy5‐Cy7) for simultaneous monitoring of reactive oxygen and nitrogen species (RONS) variations both in vitro and in vivo. HCy5‐Cy7 specifically turns on its fluorescence at 660 nm via superoxide or hydroxyl radical (O2 .−, . OH)‐mediated oxidation of reduced HCy5 moiety to Cy5, while peroxynitrite or hypochlorous species (ONOO −, ClO − )‐induced Cy7 structural degradation causes the emission turn‐off at 800 nm. Such multispectral but reverse signal responses allow multiplex manifestation of in situ oxidative and nitrosative stress events during the pathogenic and defensive processes in both bacteria‐infected macrophage cells and living mice. Most importantly, this study may also provide new perspectives for understanding the bacterial pathogenesis and advancing the precision medicine against infectious diseases. Abstract : The molecular probe HCy5‐Cy7 turns on its emission at 660 nm via superoxide or hydroxyl radical‐mediated oxidation of the reduced HCy5 moiety to Cy5, while peroxynitrite or hypochlorous species‐induced Cy7 structural degradation leads to the emission turn‐off at 800 nm. This allows the imaging of in situ oxidative and nitrosative stress events during the pathogenic and defensive processes of bacterial infection in both macrophage cells and living mice.