Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout. Issue 8 (13th July 2021)

• Record Type: Journal Article
• Authors: Ye, Xiaogang; Zhao, Shuo; Wu, Meiyu; Ruan, Jinghua; Tang, Xiaoli; Wang, Xiaoxiao; Zhong, Boxiong
• Is Part Of: FEBS open bio. Volume 11:Issue 8(2021)
• Page Start: 2304
• Page End: 2318
• Publication Date: 2021-07-13
• Subjects: CRISPR/Cas9 -- proteome -- sericin 1 -- silkworm -- transcriptome; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13239
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9‐mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild‐type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion. Abstract : CRISPR/Cas9‐mediated gene editing was successfully used to knock out the sericin 1 gene, and homozygous mutants were obtained. Comparative transcriptomic and proteomic analyses were performed on homozygous mutants and wild‐type individuals to reveal the role of sericin 1 in the defense system.