Tissue Fluid Triggered Enzyme Polymerization for Ultrafast Gelation and Cartilage Repair. Issue 36 (28th July 2021)

• Record Type: Journal Article
• Authors: Zhang, Qi; Xu, Huaxing; Wu, Chu; Shang, Yinghui; Wu, Qing; Wei, Qingcong; Zhang, Qi; Sun, Yao; Wang, Qigang
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 36(2021)
• Page Start: 19982
• Page End: 19987
• Publication Date: 2021-07-28
• Subjects: carbon Radicals -- cartilage Repair -- decarboxylation -- enzymatic polymerization -- hydrogel; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202107789
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The in situ gelation of injectable precursors is desirable in the field of tissue regeneration, especially in the context of irregular defect filling. The current driving forces for fast gelation include the phase‐transition of thermally sensitive copolymers, click chemical reactions with tissue components, and metal coordination effect. However, the rapid formation of tough hydrogels remains a challenge. Inspired by aerobic metabolism, we herein propose a tissue‐fluid‐triggered cascade enzymatic polymerization process catalyzed by glucose oxidase and ferrous glycinate for the ultrafast gelation of acryloylated chondroitin sulfates and acrylamides. The highly efficient production of carbon radicals and macromolecules contribute to rapid polymerization for soft tissue augmentation in bone defects. The copolymer hydrogel demonstrated the regeneration‐promoting capacity of cartilage. As the first example of using artificial enzyme complexes for in situ polymerization, this work offers a biomimetic approach to the design of strength‐adjustable hydrogels for bio‐implanting and bio‐printing applications. Abstract : We employ an artificial enzyme complex to trigger ultrafast hydrogelation by the tandem biocatalytic generation of carbon radicals from tissue glucose, which demonstrates a high cellular biocompatibility and a significant promotion of cartilage regeneration following the in situ injection.