Bioactive chitosan biguanidine-based injectable hydrogels as a novel BMP-2 and VEGF carrier for osteogenesis of dental pulp stem cells. (1st December 2021)

• Record Type: Journal Article
• Authors: Divband, Baharak; Aghazadeh, Marziyeh; Al-qaim, Zahraa Haleem; Samiei, Mohammad; Hussein, Falah H.; Shaabani, Alireza; Shahi, Shahriar; Sedghi, Roya
• Is Part Of: Carbohydrate polymers. Volume 273(2021)
• Publication Date: 2021-12-01
• Subjects: Chitosan hydrogel -- Bone tissue engineering -- Growth factor -- Bone scaffold; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118589
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Nowadays, vascularization and mineralization of bone defects is the main bottleneck in the bone regeneration field that is needed to be overcome and developed. Here, we prepared novel in-situ formed injectable hydrogels based on chitosan biguanidine and carboxymethylcellulose loaded with vascular endothelial growth factor (VEGF) and recombinant Bone morphogenetic protein 2 (BMP-2) and studied its influence on osteoblastic differentiation of dental pulp stem cells (DPSCs). The sequential release behavior of the VEGF and BMP-2 from hydrogels adjusted with the pattern of normal human bone growth. MTT assay exhibited that these hydrogels were non-toxic and significantly increased DPSCs proliferation. The Real-time PCR and Western blot analysis on CG11/BMP2-VEGF showed significantly higher gene and protein expression of ALP, COL1α1, and OCN. These results were confirmed by mineralization assay by Alizarin Red staining and Alkaline phosphatase enzyme activity. Based on these evaluations, these hydrogel holds potential as an injectable bone tissue engineering platform. Graphical abstract: Unlabelled Image Highlights: Novel in-situ formed injectable chitosan biguanidine hydrogel was prepared. The release behavior of the VEGF and BMP-2 consistent with the pattern of normal human bone growth The hydrogel significantly increased DPSCs proliferation. The hydrogel showed evaluated expression of ALP, COL1α1, and OCN genes and proteins.