A cell-free ROS-responsive hydrogel/oriented poly(lactide-co-glycolide) hybrid scaffold for reducing inflammation and restoring full-thickness cartilage defects in vivo. (14th September 2021)

• Record Type: Journal Article
• Authors: Wu, Xinyu; Ding, Jie; Xu, Peifang; Feng, Xue; Wang, Zhaoyi; Zhou, Tong; Tu, Chenxi; Cao, Wangbei; Xie, Jieqi; Deng, Liwen; Shen, Liyin; Zhu, Yang; Gou, Zhongru; Gao, Changyou
• Is Part Of: Biomedical materials. Volume 16:Number 6(2021)
• Publication Date: 2021-09-14
• Subjects: hydrogel -- scaffold -- ROS-responsive -- cartilage regeneration -- poly(lactide-co-glycolide); Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/ac21dd
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: The modulation of inflammation in tissue microenvironment takes an important role in cartilage repair and regeneration. In this study, a novel hybrid scaffold was designed and fabricated by filling a reactive oxygen species (ROS)-scavenging hydrogel (RS Gel) into a radially oriented poly(lactide-co-glycolide) (PLGA) scaffold. The radially oriented PLGA scaffolds were fabricated through a temperature gradient-guided phase separation and freeze-drying method. The RS Gel was formed by crosslinking the mixture of ROS-responsive hyperbranched polymers and biocompatible methacrylated hyaluronic acid (HA-MA). The hybrid scaffolds exhibited a proper compressive modulus, good ROS-scavenging capability, and cell compatibility. In vivo tests showed that the hybrid scaffolds significantly regulated inflammation and promoted regeneration of hyaline cartilage after they were implanted into full-thickness cartilage defects in rabbits for 12 w. In comparison with the PLGA scaffolds, the neo-cartilage in the hybrid scaffolds group possessed more deposition of glycosaminoglycans and collagen type II, and were well integrated with the surrounding tissue.