In Vivo Gold Complex Catalysis within Live Mice. Issue 13 (15th February 2017)

• Record Type: Journal Article
• Authors: Tsubokura, Kazuki; Vong, Kenward K. H.; Pradipta, Ambara R.; Ogura, Akihiro; Urano, Sayaka; Tahara, Tsuyoshi; Nozaki, Satoshi; Onoe, Hirotaka; Nakao, Yoichi; Sibgatullina, Regina; Kurbangalieva, Almira; Watanabe, Yasuyoshi; Tanaka, Katsunori
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 13(2017)
• Page Start: 3579
• Page End: 3584
• Publication Date: 2017-02-15
• Subjects: amide bond formation -- fluorescent labeling -- glycoalbumin -- gold catalysis; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201610273
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–Au III complex was designed and developed that enables organ‐specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5‐ and TAMRA‐linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications. Abstract : The first metal‐catalyzed reaction that proceeds within live mice is based on a targeting approach with glycans. Glycoalbumin–Au III complexes can be accumulated in specific organs where they catalyze amide bond formation between a propargyl ester probe and amine groups on nearby proteins. The selective targeting was confirmed by whole body fluorescence imaging and analysis of dissected tissues.