Comprehensive flow cytometric reference intervals of leukocyte subsets from six study centers across Europe. (3rd August 2020)

• Record Type: Journal Article
• Authors: Oras, A; Quirant-Sanchez, B; Popadic, D; Thunberg, S; Winqvist, O; Heck, S; Cwikowski, M; Riemann, D; Seliger, B; Martinez Caceres, E; Uibo, R; Giese, T
• Is Part Of: Clinical and experimental immunology. Volume 202:Number 3(2020)
• Page Start: 363
• Page End: 378
• Publication Date: 2020-08-03
• Subjects: blood leukocyte counts -- flow cytometry -- immunophenotyping -- reference intervals; Immunopathology -- Periodicals; 616.079
• DOI: 10.1111/cei.13491
• Languages: English
• ISSNs: 0009-9104

Abstract

Summary: A group of European FOCIS Centers of Excellence adapted panels of the Human Immunophenotyping Consortium (HIPC) for whole blood analysis. Using four core panels [T/regulatory T cell/B/natural killer (T/Treg /B/NK) and myeloid cells] the main leukocyte populations were analyzed in a clinical–diagnostic setting in a harmonized manner across different platforms. As a first step, the consortium presents here the absolute and relative frequencies of the leukocyte subpopulations in the peripheral blood of more than 300 healthy volunteers across six different European centers. Graphical Abstract: