A comparison of TRECs and flow cytometry for naive T cell quantification. (27th October 2017)

• Record Type: Journal Article
• Authors: Adams, S P; Kricke, S; Ralph, E; Gilmour, N; Gilmour, K C
• Is Part Of: Clinical and experimental immunology. Volume 191:Number 2(2018:Feb.)
• Page Start: 198
• Page End: 202
• Publication Date: 2017-10-27
• Subjects: CD31 -- CD45RA -- naive T cells -- TRECs; Immunopathology -- Periodicals; 616.079
• DOI: 10.1111/cei.13062
• Languages: English
• ISSNs: 0009-9104

Abstract

Summary: Assessment of thymic output by measurement of naive T cells is carried out routinely in clinical diagnostic laboratories, predominantly using flow cytometry with a suitable panel of antibodies. Naive T cell measurements can also be made using molecular analyses to quantify T cell receptor excision circle (TRECs) levels in sorted cells from peripheral blood. In this study we have compared TRECs levels retrospectively with CD45RA + CD27 + T cells and also with CD45RA + CD31 + T cells in 134 patient samples at diagnosis or during follow-up. Both panels provide naive T cell measurements that have a strongly positive correlation with TRECs numbers and are suitable for use with enumerating naive T cell levels in a clinical laboratory. Graphical Abstract: