Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71. Issue 3 (2nd March 2021)

• Record Type: Journal Article
• Authors: Jeong, Da Som; Kim, Myoung Hee; Lee, Ji‐Yeon
• Is Part Of: FEBS open bio. Volume 11:Issue 3(2021)
• Page Start: 804
• Page End: 812
• Publication Date: 2021-03-02
• Subjects: CTCF -- epigenetic modification -- pregnancy‐specific glycoproteins -- Swan 71 -- trophoblast; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13087
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Pregnancy‐specific glycoproteins (PSGs) are fetal proteins secreted by the placenta during pregnancy. The PSG level in maternal serum is an indicator of risk for pregnancy complications. However, little is known about the molecular mechanisms underlying PSG gene expression. Recently, the importance of epigenetic regulation of placental genes has been emphasized in the study of developmental defects and placental disease. In this study, the role of the CCCTC‐binding factor (CTCF) in regulation of PSG expression was investigated to better understand the epigenetic regulatory mechanisms of the PSG genes. Inhibition of CTCF expression disturbed transcription of several PSG genes: PSG1, PSG2, PSG4, PSG5, PSG8, and PSG9 were upregulated and PSG6 and PSG11 were downregulated. These transcriptional changes were correlated with decreased CTCF binding and changes in histone modification at the PSG promoters. Our data demonstrate that CTCF is a potential mediator in the regulation of PSG gene expression. Abstract : The transcriptional factor CCCTC‐binding factor (CTCF) is involved in the regulation of clustered genes. We investigated whether CTCF could regulate the pregnancy‐specific glycoprotein (PSG) family genes associated with successful pregnancy. Interestingly, the effects of CTCF silencing on the expression of PSG genes are not identical and appear to act individually for each gene. These transcriptional changes are correlated with histone modifications.