Wide-Range Mass Calibration for Negative-Ion DART Mass Spectrometry. Issue 2 (April 2014)

• Record Type: Journal Article
• Authors: Gross, Jürgen H.
• Is Part Of: European journal of mass spectrometry. Volume 20:Issue 2(2014)
• Page Start: 155
• Page End: 161
• Publication Date: 2014-04
• Subjects: Direct analysis in real time -- DART -- mass spectrometry -- Agilent ES tune mix -- phosphazene -- mass calibration -- accurate mass -- Fourier transform ion cyclotron resonance -- FT-MS -- Orbitrap; Mass spectrometry -- Periodicals; Mass Spectrometry; Mass spectrometry; Periodicals; Periodicals; 543.6505
• DOI: 10.1255/ejms.1266
• Languages: English
• ISSNs: 1469-0667

Abstract

A method for accurate mass calibration covering a wide m/z range in negative-ion direct analysis in real time mass spectrometry (DART-MS) is described. It is based on a commercially available tuning solution (Agilent ES tune mix) normally applied in electrospray ionization. This acetonitrile solution is composed of ammonium trifluoroacetate, betaine, 2, 4, 6-tris(heptafluoropropyl)-1, 3, 5-triazine, and various symmetrical hexakis-(fluoroalkoxy)-phosphazenes. Upon addition of 2% (v/v) trifluoroacetic acid in negative-ion DART-MS, this mixture yields negative ions covering a range of m/z 69 to m/z 2834. Validity of the calibration procedure is demonstrated on a Fourier transform ion cyclotron resonance mass spectrometer by application to polyethylene glycol diacid and [60]fullerene. Experimental conditions and a mass reference list are provided.