One-pot fabrication of sacchachitin for production of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) utilized as scaffolds to enhance bone regeneration. (15th February 2021)

• Record Type: Journal Article
• Authors: Wu, Meng-Huang; Wang, Weu; Chao, Fang-Ching; Hsieh, Chien-Ming; Chen, Ling-Chun; Lin, Hong-Liang; Ho, Hsiu-O; Huang, Tsung-Jen; Sheu, Ming-Thau
• Is Part Of: Carbohydrate polymers. Volume 254(2021)
• Publication Date: 2021-02-15
• Subjects: Acetic acid -- Citric acid -- Hydrogen peroxide solution (30 wt.% H2O2) -- Potassium hydroxide -- Sodium bromide -- Sodium chlorite (NaClO2) -- Sodium hypochlorite -- 2, 2, 6, 6-Tetramethyl-1-piperidinyloxy (TEMPO) -- Thiazolyl blue tetrazolium bromide (MTT reagent); Ganoderma -- Sacchachitin nanofibers -- One-pot fabrication -- TEMPO-oxidation -- Bone regeneration; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117270
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: One-pot fabrication of sacchachitin (SC) for mass-production was successfully developed and optimized. Overall yield of one-pot-fabricated SC was up to 35 %w/w of initial weight. So-obtained SC with fibrous texture soft enough for mechanical disintegration into SC nanofibers (SCNFs). So-obtained SCs showed better dispersion for producing TEMPO-oxidized SCNFs (T033SC). T033SC formed 3D gelatinous scaffold with higher calcium-trapping ability provided excellent bone regeneration environment. Abstract: One-pot fabrication of sacchachitin (SC) for mass-production was developed and optimized by selecting KOH as alkaline agent in depigmentation step and utilizing NaClO2 as bleaching agent in subsequent step in the same pot. Overall yield of one-pot-fabricated SC was up to 35 %w/w of initial weight with a fibrous texture soft enough for mechanical disintegration into SC nanofibers (SCNFs) and better dispersion for producing TEMPO-oxidized SCNFs (T033SC). Both SCNFs and T033SC could form a 3D gelatinous scaffold into which MC3T3-E1 cells were attracted. Higher calcium-trapping ability of T033SC resulting from a greater extent of carboxylate groups provided an excellent bone regeneration environment that resulted in better outcomes of bone regeneration in a femur defect rat model compared to those with SCNFs possessed fewer carboxylate groups. In conclusion, biomaterial scaffolds based on TEMPO-oxidized SCNFs produced from one-pot fabricated SC showed great potential for bone regeneration due to unique physical and chemical properties.