Purification of cell‐derived Japanese encephalitis virus by dual‐mode chromatography. (11th June 2020)

• Record Type: Journal Article
• Authors: Zhang, Fuliang; Luo, Jun; Teng, Man; Xing, Guangxu; Guo, Junqing; Zhang, Yihua
• Is Part Of: Biotechnology and applied biochemistry. Volume 68:Number 3(2021)
• Page Start: 547
• Page End: 553
• Publication Date: 2020-06-11
• Subjects: CaptoTM Core 700 -- Japanese encephalitis virus -- purification -- Sepharose 4 fast flow -- tangential‐flow filtration; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1960
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time‐consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and Capto TM Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large‐scale production of high‐purity JEV. Abstract : Comparison of different purification mehods of Japanese Encephalitis Virus. A rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography and CaptoTM Core 700 chromatography. The purified virus particles maintained good activity and immunogenicity.