Nanocage‐Based Capture‐Detection System for the Clinical Diagnosis of Autoimmune Disease. Issue 25 (24th May 2021)

• Record Type: Journal Article
• Authors: Zhang, Yanan; Li, Yingni; Zhang, Jianlin; Chen, Xuehui; Zhang, Ruofei; Sun, Guoming; Jiang, Bing; Fan, Kelong; Li, Zhanguo; Yan, Xiyun
• Is Part Of: Small. Volume 17:Issue 25(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-24
• Subjects: binding affinity -- capture and detection system -- peptides displaying -- primary Sjögren's syndrome diagnosis -- self‐assembly nanocage; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202101655
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: The detection of autoantibodies is critical for diagnosis of autoimmune diseases. However, the sensitivity is often limited by the properties of the antigens and the detection systems such as enzyme‐linked immunosorbent assay (ELISA). Here, employing the multidisplay ability of ferritin, a highly sensitive nanocage‐based capture‐detection system is designed, of which the sensitivity is 100–1000‐fold higher than that of conventional ELISA methods. The capture nanocages are constructed by displaying the primary Sjögren's syndrome (pSS)‐related antigenic peptides on ferritin nanocage, which present epitopes effectively and high affinity, leading to tenfold higher capture capability for autoantibodies. Human IgG Fc‐binding peptides are also engineered on ferritin nanocage, which enable high binding affinity and efficient horseradish peroxidase (HRP)‐labeling. Compared with commercial HRP‐conjugated anti‐human IgG antibody, the nanocage‐based detecting probe exhibited more than tenfold increased sensitivity. Autoantibodies are then examined in 91 sera from patients with pSS, 51 from rheumatoid arthritis, 54 from systemic lupus erythematosus, and 55 from healthy individuals by using the nanocage‐based ELISA. The results indicate that the nanocage‐based capture‐detection system is an effective detection platform and provide a novel and more sensitive method for the diagnosis of autoimmune diseases. Abstract : Capture probes and detection probes are constructed by displaying antigen and anti‐Fc peptides on the surface of ferritin nanocage. Multimeric display of peptides significantly enhances their binding with corresponding antibodies and improves the detecting sensitivity 100–1000‐fold higher than that of conventional enzyme‐linked immunosorbent assay methods. Nanocage‐based capture‐detection system is a highly sensitive platform for diagnosis of autoimmune disease.