Cells which contain S-100 protein in Hodgkin's disease: a quantitative study. Issue 9 (September 1985)

• Record Type: Journal Article
• Authors: Sangster, G; Crocker, J; Jenkins, R; Leyland, M J
• Is Part Of: Journal of clinical pathology. Volume 38:Issue 9(1985)
• Page Start: 1021
• Page End: 1024
• Publication Date: 1985-09
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.38.9.1021
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Paraffin sections from a series of 50 lymph nodes affected by Hodgkin's disease were examined by means of the unlabelled primary antibody peroxidase-antiperoxidase method to detect those cells which contained S-100 protein. In addition, 15 lymph nodes showing reactive follicular hyperplasia were studied. A simple enumeration procedure (eyepiece graticule) was used to count the number of such cells in 20 standard 25 X microscope fields. In the specimens of nodular sclerosing Hodgkin's disease many cells positive for S-100 protein were present, in contrast to the other Rye subtypes, which showed a relative paucity. By comparison, the lymph nodes showing reactive follicular hyperplasia contained a similar number of cells containing S-100 to those seen in the nodular sclerosing lymph nodes affected by Hodgkin's disease.