DNA Nanolithography Enables a Highly Ordered Recognition Interface in a Microfluidic Chip for the Efficient Capture and Release of Circulating Tumor Cells. (4th June 2020)

• Record Type: Journal Article
• Authors: Zhang, Jialu; Lin, Bingqian; Wu, Lingling; Huang, Mengjiao; Li, Xingrui; Zhang, Huimin; Song, Jia; Wang, Wei; Zhao, Gang; Song, Yanling; Yang, Chaoyong
• Is Part Of: Angewandte Chemie. Volume 132:Number 33(2020)
• Page Start: 14219
• Page End: 14223
• Publication Date: 2020-06-04
• Subjects: aptamers -- circulating tumor cells -- DNA nanostructures -- microfluidics -- nanolithography; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202005974
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Microfluidic chips with nano‐scale structures have shown great potential, but the fabrication and cost issues restrict their application. Herein, we propose a conceptually new "DNA nanolithography in a microfluidic chip" by using sub‐10 nm three‐dimensional DNA structures (TDNs) as frameworks with a pendant aptamer at the top vertex (ApTDN‐Chip). The nano‐scale framework ensures that the aptamer is in a highly ordered upright orientation, avoiding the undesired orientation or crowding effects caused by conventional microfluidic interface fabrication processes. Compared with a monovalent aptamer modified chip, the capture efficiency of ApTDN‐Chip was enhanced nearly 60 % due to the highly precise dimension and rigid framework of TDNs. In addition, the scaffolds make DNase I more accessible to the aptamer with up to 83 % release efficiency and 91 % cell viability, which is fully compatible with downstream molecular analysis. Overall, this strategy provides a novel perspective on engineering nano‐scaffolds to achieve a more ordered nano‐topography of microfluidic chips. Abstract : Catching cells with DNA : A conceptually new strategy is proposed for engineering a microfluidic chip using sub‐10 nm three‐dimensional DNA structures (TDNs) with a pendant aptamer at the top vertex (ApTDN‐Chip). At the microfluidic nanointerface, the improvement of effective recognition by the aptamers is ascribed to the more ordered nano‐topography.