Analysis of lymphocyte subsets of bone marrow in patients with rheumatoid arthritis by two colour immunofluorescence and flow cytometry. Issue 3 (March 1990)

• Record Type: Journal Article
• Authors: Doita, M; Maeda, S; Kawai, K; Hirohata, K; Sugiyama, T
• Is Part Of: Annals of the rheumatic diseases. Volume 49:Issue 3(1990)
• Page Start: 168
• Page End: 171
• Publication Date: 1990-03
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/ard.49.3.168
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Lymphocyte subsets defined by monoclonal antibodies were investigated in bone marrow and peripheral blood of 17 patients with rheumatoid arthritis (RA); 13 patients with osteoarthritis or aseptic necrosis served as controls. Patients with RA were found to have a raised OKT 4/8 ratio both in bone marrow and peripheral blood in comparison with the controls. Furthermore, bone marrow of RA showed a lower percentage of OKT 8+ T cells than that of controls. The percentage of HLA-DR+ T cells was higher in bone marrow than in peripheral blood of RA, though a slightly lower percentage was detected in bone marrow than in peripheral blood of controls. Thus T cell subsets in bone marrow of RA differ significantly from those of controls. Patients with RA had a higher OKT 4/8 ratio and a higher percentage of HLA-DR+ T cells in bone marrow than controls, suggesting that T cell subsets in bone marrow of RA are in an immunologically activated state and that T cell subsets are affected by rheumatoid inflammation in bone marrow of RA.