Enhanced oral bioavailability and neuroprotective effect of fisetin through its SNEDDS against rotenone-induced Parkinson's disease rat model. (October 2020)

• Record Type: Journal Article
• Authors: Kumar, Rajan; Kumar, Rakesh; Khurana, Navneet; Singh, Sachin Kumar; Khurana, Shelly; Verma, Surajpal; Sharma, Neha; Kapoor, Bhupinder; Vyas, Manish; Khursheed, Rubiya; Awasthi, Ankit; Kaur, Jaskiran; Corrie, Leander
• Is Part Of: Food and chemical toxicology. Volume 144(2020)
• Publication Date: 2020-10
• Subjects: SNEDDS -- Fisetin -- Parkinson's disease -- Rotenone -- Bioavailability -- Alfa synuclein; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2020.111590
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Fisetin (FS) was reported to have various pharmacological activities. But due to its lower aqueous solubility and oral bioavailability, it is not in much use. As solubility and bioavailability plays and important role in the pharmacological activity, in this research work we tried to improve the oral bioavailability of fisetin. In this research work, we developed self-nanoemulsifying drug delivery system (SNEDDS) of fisetin. Developed SNEDDS were subjected for pharmacokinetic and pharmacodynamics studies against rotenone-induced Parkinson's disease (PD) model in rats. Higher Cmax and area under the curve during pharmacokinetic study indicated that SNEDDS improved the oral bioavailability of FS and also increased the mean residence time of drug in plasma. Results of behavior parameters (locomotor, muscle co-ordination and catalepsy), biochemical estimation (TBARS, nitrite, GSH, SOD and CAT) and ELISA (soluble alfa synuclein, BDNF, TNF-α and IL-6) confirmed the significantly improved (p < 0.05) neuroprotection in rats treated with FS loaded SNEDDS as compared to rats treated with naïve FS. This study suggests that SNEDDS improved the oral bioavailability of FS which further helped in improving its neuroprotective activity in rat model of PD. It further suggests the potential use of FS-SNEDDS in effective management of PD condition. Graphical abstract: Image 1 Highlights: FS-SNEDDS improved the oral bioavailability of fisetin. FS-SNEDDS provided significant neuroprotection against rotenone induced neurotoxicity. Significant attenuation of alfa synuclein's aggregation was noted for FS-SNEDDS. Excellent correlation was noted between PK and PD results for FS-SNEDDS.