Regulation of taurine in OTA-induced apoptosis and autophagy. (15th July 2020)

• Record Type: Journal Article
• Authors: Liu, Kai; Mao, Xinru; Li, Hu; Liu, Dandan; Le, Guannan; Gan, Fang; Pan, Cuiling; Huang, Kehe; Chen, Xingxiang
• Is Part Of: Toxicon. Volume 181(2020)
• Page Start: 82
• Page End: 90
• Publication Date: 2020-07-15
• Subjects: Ochratoxin A -- PK-15 -- Taurine -- Apoptosis -- Autophagy; Tau Taurine -- OTA Ochratoxin A -- DMSO Dimethyl sulphoxide -- BCA Bicinchoninic acid -- ECL Enhanced chemiluminescence -- DAPI 6-diamidino-2-phenylindole -- DMEM Dulbecco's modified Eagle's medium -- OD Optical Density -- NBCS Newborn calf serum -- MTT 3-(4, 5-cimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide -- LDH Lactate Dehydrogenase -- PBS Phosphate-buffered saline -- SDS-PAGE sodium salt-Polyacrylamide gel electrophoresis -- RIPA Radio Immunoprecipitation Assay -- PMSF Phenylmethylsulfonyl Fluoride -- PVDF Polyvinylidene fluoride -- TBST Tris-buffered saline with Tween -- ROS reactive oxygen species -- ANOVA One-way analysis of variance -- SEM Standard error mean -- GFP-LC3 green fluorescent protein-microtubule-associated protein 1 light-chain 3; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2020.04.097
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Ochratoxin A (OTA), one of the most deleterious mycotoxins, could cause a variety of toxicological effects especially nephrotoxicity in animals and humans. Taurine, a wide-distributed cytoprotective amino acid, plays an important role as a basic factor for maintaining cellular integrity homeostasis. However, the potential effect of taurine in OTA-induced nephrotoxicity remains unknown. In the present study, we demonstrated that OTA treatment at 4.0–8.0 μM increased apoptosis in PK-15 cells as shown by increased the ratio of apoptosis and protein expression of Bax and cleaved-caspase-3, decreased protein expression of Bcl-2. Meantime, OTA treatment triggered autophagy, as indicated by markedly increased the protein expression of LC3-II and fluorescence intensity of GFP-LC3 dots. Taurine supplementation decreased OTA-induced cytotoxicity and attenuated apoptosis as shown by the decreased Annexin V/PI staining and the decreased expression of apoptosis-related proteins including Bax and caspase-3. Meanwhile, taurine attenuated OTA-induced autophagy by decreased the protein expression of LC3-II and fluorescence intensity of GFP-LC3 dots to maintain cellular homeostasis. In conclusion, taurine treatment could alleviate OTA-induced apoptosis and inhibit the triggered autophagy in PK-15 cells. Our study provides supportive data for the potential roles of taurine in reducing OTA-induced renal toxicity. Highlights: Taurine supplementation protected PK-15 cells against OTA-induced cytotoxicity. Taurine supplementation alleviated OTA-induced PK-15 cells apoptosis. Taurine supplementation attenuated the autophagy of PK-15 cells induced by OTA.