Detection of Acinetobacter pittii ST220 co-producing NDM-1 and OXA-820 carbapenemases from a hospital sink in a non-endemic country of NDM. (June 2020)

• Record Type: Journal Article
• Authors: Iimura, Masaki; Hayashi, Wataru; Arai, Eriko; Natori, Tatsuya; Horiuchi, Kazuki; Matsumoto, Go; Tanaka, Hayato; Soga, Eiji; Nagano, Yukiko; Nagano, Noriyuki
• Is Part Of: Journal of global antimicrobial resistance. Volume 21(2020)
• Page Start: 353
• Page End: 356
• Publication Date: 2020-06
• Subjects: Acinetobacter pittii -- blaNDM-1 -- blaOXA-820 -- Hospital sink -- ST220 -- Biofilm-associated genes; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2019.11.013
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: This was the first report of Acinetobacter pittii ( A. pittii ) co-harbouring bla NDM-1 and bla OXA-820 in Japan. A. pittii was isolated from a hospital sink with no history of New Delhi metallo-β-lactamase detection. A. pittii ST220 had resistance genes bla NDM-1, bla OXA-820, bla ADC-43, and aphA6 . The bla NDM-1 preceded by IS Aba125 was located on the 41, 022-bp plasmid. Abstract: Objectives: NDM-1 is by far one of the most commonly prevalent carbapenemases in Enterobacteriaceae and Acinetobacter baumannii . This study presented an Acinetobacter pittii ( A. pittii ) isolate co-harboring bla NDM-1 and bla OXA-820 from a university hospital sink, where New Delhi metallo-β-lactamase (NDM) producers have not been found in either patients or their environments. Methods: Whole-genome sequencing was performed on the HiSeq 4000 platform, and the reads were de novo assembled using the A5-miSeq Assembly pipeline. Annotation of the resulting scaffolds were performed by using the DDBJ Fast Annotation and Submission Tool (DFAST). The bla NDM-1 -carrying plasmid was determined. Results: The A. pittii ST220 strain SU1805 detected from a sink strainer in the treatment room was resistant to imipenem and meropenem. Antimicrobial resistance genes bla NDM-1, bla OXA-820, bla ADC-43, and aphA6 were found in this strain. The bla NDM-1 was found to be located downstream of an IS Aba125 element on a plasmid pSU1805NDM with a size of 41, 022 bp, and GC content of 38.3% harbouring 48 protein-coding genes. The aphA6 gene was also located upstream of the IS Aba125 on the same plasmid. The A. pittii intrinsic bla OXA-213 -like gene bla OXA-820 was located between fxsA and yncA genes in the chromosome. The strain also harboured biofilm-associated genes such as ompA, the csu operon and their regulating genes bfmRS . Conclusion: This study described the first isolation of NDM-1-producing A. pittii in Japan, and highlighted the importance of proper implementation of measures against AMR for sink drainage systems, since NDM producers may have already been hidden in such environments in a non-endemic country of NDM.