Dietary infection of Enterobacter ludwigii causes fat accumulation and resulted in the diabetes-like condition in Drosophila melanogaster. (December 2020)

• Record Type: Journal Article
• Authors: Priyadarsini, Subhashree; Mukherjee, Sumit; Samikshya, S.N.; Bhanja, Amrita; Paikra, Sanjeev Kumar; Nayak, Nibedita; Mishra, Monalisa
• Is Part Of: Microbial pathogenesis. Volume 149(2020)
• Publication Date: 2020-12
• Subjects: Enterobacter ludwigii -- Lipid -- Trehalose -- Triglyceride -- Insulin-like peptide -- Obesity -- Diabetes; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104276
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Bacteria as a foreign pathogen can alter the physiology and metabolism of the host. Many of the teeth infecting bacteria known to be associated with obesity and diabetes in various organisms. The current study aims to check the effect of teeth infecting bacteria Enterobacter ludwigii on model organism Drosophila melanogaster . To check the effect, various concentrations of E. ludwigii on fly physiology the bacteria were added to the fly food. Flies were allowed to grow and colonise in infected food. The offsprings were checked for the accumulation of lipid and fat. With the increase of bacteria within the gut the amount of lipid and fat increases. Alongwith the fat various biochemical parameters like glucose, trehalose, protein and triglyceride level found to be altered. Within the fly gut various metals, which have a role in the metabolism is altered. However, during colonisation within the gut, the morphology of the bacteria remains unaltered. In the adult fly, all the biochemical parameters like glucose, trehalose, protein and triglyceride level increased. The expression level of Dilp is upregulated. Altogether, the current study reports an infection of E. ludwigii causes the accumulation of fat and alters glucose metabolism in Drosophila melanogaster . Graphical abstract: Image 1 Highlights: An infection of E.ludwigii causes accumulation of lipid and fat in 3rd inster larval gut of Drosophila . An elevated level of glucose, trehalose, triglyceride, and protein was observed in both adults and larvae. Dilp level is upregulated and the weight of the fly decreases after more colonisation of the bacteria. Metals associated with the metabolic pathways are altered within the gut. E.ludwigii causes fat accumulation within the gut and alter glucose metabolism in Drosophila melanogaster.