Human Cytomegalovirus: Propagation, Quantification, and Storage. Issue 1 (1st August 2010)

• Record Type: Journal Article
• Authors: Britt, William J.
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 18:Issue 1(2010)
• Page Start: 14E.3.1
• Page End: 14E.3.17
• Publication Date: 2010-08-01
• Subjects: human cytomegalovirus -- virus propagation -- virus quantification; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/9780471729259.mc14e03s18
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Human cytomegalovirus (HCMV) is the largest and perhaps the most structurally complex member of the family of human herpesviruses. It is the prototypic virus of the β‐herpesvirus subfamily. As with other cytomegaloviruses, HCMV is exquisitely species specific and undergoes lytic replication only in cells of human origin. In addition, its replication is limited almost entirely to primary cells and a limited number of transformed cell lines. Together with its prolonged replicative cycle of ∼48 hr, the propagation and quantification of HCMV can present technical challenges. In this brief set of protocols, the propagation of laboratory strains of HCMV and their quantitation is described. In a third series of protocols, the concentration and gradient purification of HCMV for more specialized downstream applications is described. Curr. Protoc. Microbiol . 18:14E.3.1‐14E.3.17. © 2010 by John Wiley & Sons, Inc.