Molecular detection of Cryptosporidium spp. and Enterocytozoon bieneusi in Longjiang Wagyu cattle in Northeastern China. (December 2020)

• Record Type: Journal Article
• Authors: Xue, Nian-Yu; Liu, Feng; Tao, Wei-Fu; Zhao, Quan; Qiu, Hong-Yu; Hu, Yang; Chen, Yu; Wei, Xin-Yu; Wang, Wei; Gao, Duo; Ni, Hong-Bo; Wang, Chun-Ren
• Is Part Of: Microbial pathogenesis. Volume 149(2020)
• Publication Date: 2020-12
• Subjects: Cryptosporidium spp. -- Enterocytozoon bieneusi -- Longjiang wagyu cattle -- Molecular detection -- China; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104526
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Cryptosporidium spp. and Enterocytozoon bieneusi are two important zoonotic pathogens that can cause diarrhea and other gastrointestinal illnesses in humans and animals. However, the prevalence and genotype of the parasites in Longjiang Wagyu cattle in Heilongjiang Province, Northeast China have not been reported. In the present study, a total of 423 fecal samples of Longjiang Wagyu cattle collected from different farms in Heilongjiang Province, Northeast China, were examined for Cryptosporidium spp. and E. bieneusi using nested PCR. The overall infection rates for Cryptosporidium spp. and E. bieneusi were 6.38% (n = 27) and 7.09% (n = 30), respectively. The prevalence in different age groups ranged from 3.80% (95% confidence interval (CI) 1.01–6.59) to 8.36% (95% CI 4.83–11.90) for Cryptosporidium spp. and 5.97% (95% CI 2.52–9.43) to 7.94% (95% CI 4.49–11.40) for E. bieneusi . By analyzing the DNA sequences of the small subunit (SSU) rRNA gene, two Cryptosporidium species were detected in this study, namely C. parvum (n = 25) and C. ryanae (n = 2) . The IIdA20G1 subtype was further identified by using the 60-kDa glycoprotein ( gp60 ) gene of C. parvum . E. bieneusi was identified using three known sequences through the analysis of internal transcribed spacer (ITS) sequences: J (n = 23), I (n = 5), and BEB4 (n = 2), and all belonged to group 2. The results indicated that some of the Cryptosporidium species and E. bieneusi genotypes identified in Longjiang Wagyu cattle in the study areas might have zoonotic potential. Highlights: This is a significant record of Cryptosporidium spp. and E. bieneusi in Longjiang Wagyu cattle in Heilongjiang Province China. Some of Cryptosporidium species and E. bieneusi genotypes obtained from Longjiang Wagyu cattle may have zoonotic potential. These findings will provide useful data for control of protozoan infection in Longjiang Wagyu cattle and/or other hosts.