Feeding behavior toxicity in the marine rotifer Brachionus plicatilis caused by 2, 2′, 4, 4′-tetrabromodiphenyl ether (BDE-47): Characteristics and mechanisms. (May 2021)

• Record Type: Journal Article
• Authors: Yang, Yingying; Jian, Xiaoyang; Tang, Xuexi; Ma, Wenqian; Sun, Zijie; Zhang, Xin; Fang, Kuan; Zhang, Xinxin
• Is Part Of: Chemosphere. Volume 271(2021)
• Publication Date: 2021-05
• Subjects: PBDEs -- Rotifer -- Ingestion -- Digestive performance -- Mitochondria -- AChE; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.129512
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Polybrominated diphenyl ether contamination in marine environments has received special attention due to its accumulation and magnification in the marine food web and toxicity to organisms. In the present study, a series of short-term toxicological tests were conducted with the marine rotifer Brachionus plicatilis to assess the effects on ingestion and digestive performance after treatment with 2, 2′, 4, 4′-tetrabromodiphenyl ether (BDE-47) at nonlethal concentrations under controlled laboratory conditions and to analyze the possible mechanism. The results showed that with accumulation in rotifers, BDE-47 caused a significant decline in the filtration and feeding rates in a concentration-dependent manner. Moreover, the activities of amylase (AMS) and protease were affected, indicating that BDE-47 impaired ingestion and digestion efficiency. BDE-47 exposure did not lead to abnormal microstructures in the main digestive tract (e.g., cilia around the corona, mastax, stomach, digestive gland and esophagus), but the gastric parietal cells shrank, suggesting nutritional deficiency. BDE-47 prominently induced the occurrence of irregular mitochondria at the cilia root, and mitochondrial and isocitrate dehydrogenase activity declined, indicating mitochondrial dysfunction. Furthermore, the activity of ATPase, which catalyzes ATP hydrolysis, decreased as the BDE-47 concentration rose, implying that BDE-47 retarded rotifer ATP dynamics, inevitably interfering with cilia movement to ingest food. Additionally, a significant decline in acetylcholine esterase activity was observed, which led to a hindrance in neurotransmission involved in food intake and digestion. Altogether, our results demonstrated that nonlethal concentrations of BDE-47 could induce feeding depression in rotifers, which is mainly attributed to stymied energy metabolism and nerve conduction. Graphical abstract: Image 1 Highlights: BDE-47 induced the food-intake and digestive suppression on B. plicatilis. Atrophic cells of stomach wall implied a status of nutritional deficiency. ATPase activities inhibition and mitochondrial dysfunction hampered cilia movement. The decline of AChE activity also contributed to this inhibition.