3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells. (October 2020)

• Record Type: Journal Article
• Authors: Lu, Jing; Lu, Jianing; Chen, Yan; Feng, Zhe; Liu, Shuang; Guan, Shuang
• Is Part Of: Food and chemical toxicology. Volume 144(2020)
• Publication Date: 2020-10
• Subjects: 3-MCPD -- Autophagic flux -- Lysosome -- TFEB; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2020.111575
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: 3-chloro-1, 2-propanediol (3-MCPD) is a well-known contaminant that was produced in the thermal processing of food. Dietary intake represents the greatest source of exposure to 3-MCPD. Autophagy is an important catabolic pathway that plays an important role in liver physiological function. Evidence suggests that 3-MCPD exposure causes toxicity in liver, but the mechanism remains unknown. Here, we explored the effects of 3-MCPD on autophagic flux and traced the molecular mechanism in HepG2 cells. The data showed 3-MCPD exposure promoted the accumulation of autophagosomes in HepG2 cells. Subsequently, by detected te expression of LC3-Ⅱ and P62 and transfection of mRFP-GFP-LC3 adenovirus, we found that the accumulation of autophagosomes was caused by inhibition of autophagic flux. After that, we investigate lysosomal function and found that 3-MCPD induced lysosomal alkalinization. Further, we detected the expression of TFEB, which is a key nuclear transcription factor in control of lysosome biogenesis and function. We found that 3-MCPD inhibited the nuclear expression of TFEB and mRNA levels of some target genes of TFEB. In order to further verify the role of TFEB in autophagic flux blockage in HepG2 cells induced by 3-MCPD, we overexpressed TFEB by transfection with adenovirus and found that both autophagy inhibition and lysosomal alkalization induced by 3-MCPD were alleviated. These results suggested that 3-MCPD could induce the autophagic flux blockage in HepG2 cells. The possible mechanism was due to the destruction of lysosomal function. Highlights: 3-MCPD inhibited autophagic flux in HepG2 cells. 3-MCPD could not inhibit the fusion of autophagosomes and lysosomes. 3-MCPD decreased pH value of lysosomal. 3-MCPD inhibited transcription of TFEB and its target genes. Overexpression of TFEB could restore autophagy flux blockage and lysosomal alkalinization induced by 3-MCPD.