Aflatoxin B1 degradation by microorganisms isolated from Kombucha culture. (May 2020)

• Record Type: Journal Article
• Authors: Ben Taheur, Fadia; Mansour, Chalbia; Ben Jeddou, Khawla; Machreki, Yasmine; Kouidhi, Bochra; Abdulhakim, Jawaher Abdualbaqi; Chaieb, Kamel
• Is Part Of: Toxicon. Volume 179(2020)
• Page Start: 76
• Page End: 83
• Publication Date: 2020-05
• Subjects: Biodegradation -- Kombucha -- Aflatoxins B1 -- Pichia occidentalis -- Candida sorboxylosa -- Hanseniaspora opuntiae; AFB1 Aflatoxin B1 -- Hep2 Human epithelial type 2 cells -- LAB Lactic acid bacteria -- MRS Man Rogosa Sharpe -- RPMI Roswell Park Memorial Institute medium -- YPD yeast extract peptone dextrose; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2020.03.004
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Aflatoxin B1 (AFB1) is the most harmful mycotoxin. Aflatoxin occurrence in tea makes this beverage unsuitable for consumption and presented risks to human health. Therefore, researches in aflatoxin microbial degradation are necessary to overcome this problem. Kombucha beverage is associated with health promoting effects. Thus, novel strains (Lactic acid bacteria and yeasts) were isolated from a Kombucha culture and assessed for AFB1 degradation in the liquid medium (Man Rogosa and Sharpe broth, yeast extract peptone dextrose broth and black tea). The main strains involved in AFB1 decontamination were identified based on DNA sequencing and the toxicity of the new products was evaluated on Hep2 cells and on Brine shrimp ( Artemia salina ). Our results showed that after 7 days of fermentation, kombucha was able to degrade 97% of AFB1 in black tea. Moreover, the effective yeasts present in Kombucha were identified as Pichia occidentalis, Candida sorboxylosa and Hanseniaspora opuntiae and the highest AFB1 degradation capacity was accorded to P. occidentalis (59%) when cultivated in black tea. Data on cytotoxicity tests on Hep2 cells and Brine shrimp ( Artemia salina ) showed that the biodegraded products were less toxic than pure AFB1. These findings suggest that, kombucha isolated strains could be potential candidates for application in the food and feed industry with a potential aflatoxin B1 detoxification properties. Highlights: Kombucha isolates (LAB and yeasts) showed a higher AFB1 degradation in black tea. The main strains were identified as P. occidentalis, C. sorboxylosa and H. opuntiae . Pichia occidentalis degraded efficiently AFB1 (59%) when cultivated in black tea. The biodegraded byproduct was less toxic than AFB1.