Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy. Issue 31 (27th July 2022)

• Record Type: Journal Article
• Authors: Manu, V. S.; Olivieri, Cristina; Pavuluri, KowsalyaDevi; Veglia, Gianluigi
• Is Part Of: Physical chemistry chemical physics. Volume 24:Issue 31(2022)
• Page Start: 18477
• Page End: 18481
• Publication Date: 2022-07-27
• Subjects: Chemistry, Physical and theoretical -- Periodicals; 541.3
• DOI: 10.1039/d2cp01744j
• Languages: English
• ISSNs: 1463-9076

Abstract

Abstract : Water devoid RF pulses designed by an evolutionary algorithm and AI improve solvent suppression and increase the NMR spectra sensitivity. Abstract : Water suppression is of paramount importance for many biological and analytical NMR spectroscopy applications. Here, we report the design of a new set of binomial-like radio frequency (RF) pulses that elude water irradiation while exciting or refocusing the remainder of the 1 H spectrum. These pulses were generated using a combination of an evolutionary algorithm and artificial intelligence. They display higher sensitivity relative to classical water suppression schemes and tunable water selectivity to avoid suppressing 1 H resonances near the water signal. The broad bandwidth excitation obtained with these RF pulses makes them suitable for several NMR applications at high and ultra-high-field magnetic fields.