Human herpesvirus 6 latently infects mononuclear cells but not liver tissue. Issue 1 (1st January 1999)

• Record Type: Journal Article
• Authors: Yoshikawa, T; Suzuki, K; Ihira, M; Furukawa, H; Suga, S; Iwasaki, T; Kurata, T; Asonuma, K; Tanaka, K; Asano, Y
• Is Part Of: Journal of clinical pathology. Volume 52:Issue 1(1999)
• Page Start: 65
• Page End: 67
• Publication Date: 1999-01-01
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.52.1.65
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIM: To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. METHODS: Peripheral blood and liver tissue were collected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. RESULTS: Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. CONCLUSIONS: HHV-6 variant B latently infects mononuclear cells but not liver tissue.