Miniaturized Chemical Tags for Optical Imaging. Issue 34 (21st July 2022)

• Record Type: Journal Article
• Authors: Benson, Sam; de Moliner, Fabio; Tipping, William; Vendrell, Marc
• Is Part Of: Angewandte Chemie international edition. Volume 61:Issue 34(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-07-21
• Subjects: Bioconjugates -- Fluorophores -- Probes -- Raman Spectroscopy; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202204788
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Recent advances in optical bioimaging have prompted the need for minimal chemical reporters that can retain the molecular recognition properties and activity profiles of biomolecules. As a result, several methodologies to reduce the size of fluorescent and Raman labels to a few atoms (e.g., single aryl fluorophores, Raman‐active triple bonds and isotopes) and embed them into building blocks (e.g., amino acids, nucleobases, sugars) to construct native‐like supramolecular structures have been described. The integration of small optical reporters into biomolecules has also led to smart molecular entities that were previously inaccessible in an expedite manner. In this article, we review recent chemical approaches to synthesize miniaturized optical tags as well as some of their multiple applications in biological imaging. Abstract : This Minireview covers some of the recent advances in the development of small chemical tags for optical bioimaging, from their chemical synthesis and characterization to their application in different biological systems, such as cells, tissues and whole organisms. It focuses on the design of fluorescent and Raman labels and features examples of metabolites, amino acids, nucleic acids as well as organelle and cell‐targeting probes.