Polysaccharides from Lyophyllum decastes reduce obesity by altering gut microbiota and increasing energy expenditure. (1st November 2022)

• Record Type: Journal Article
• Authors: Wang, Tao; Han, Junjie; Dai, Huanqin; Sun, Jingzu; Ren, Jinwei; Wang, Wenzhao; Qiao, Shanshan; Liu, Chang; Sun, Li; Liu, Shuangjiang; Li, Dianpeng; Wei, Shenglong; Liu, Hongwei
• Is Part Of: Carbohydrate polymers. Volume 295(2022)
• Publication Date: 2022-11-01
• Subjects: LDP Lyophyllum decastes polysaccharide -- L. decastes Lyophyllum decastes -- sWAT subcutaneous white adipose tissue -- eWAT epididymal white adipose tissue -- mWAT mesenteric white adipose tissue -- T-CHO total cholesterol -- TG triglyceride -- LDL-C low-density lipoprotein cholesterol -- HFD high-fat diet -- ELISA enzyme-linked immunosorbent assay -- AST aspartate aminotransferase -- ALT alanine aminotransferase -- IL-1β interleukin-1 beta -- IL-6 interleukin-6 -- TNF-α tumor necrosis factor alpha -- LPS lipopolysaccharide -- qRT-PCR quantitative real-time PCR -- GC–MS gas chromatography–mass spectrometry -- LDA linear discriminant analysis -- LEfSe linear discriminant analysis effect size -- OTU operational taxonomic unit -- PCA principal component analysis -- THDCA taurohyodeoxycholic acid -- THCA taurohyocholic acid sodium salt -- 7-keto LCA 7-Ketolithocholic acid -- NorCA norcholic acid -- TDCA taurodeoxycholic acid -- HDCA hyodeoxycholic acid -- GHCA glycohyocholic acid -- 23norDCA 23-Nordeoxycholic acid -- iso LCA isolithocholic acid -- 12-keto LCA 12-ketolithocholic acid -- allo LCA allolithocholic acid -- LCA-S lithocholic acid 3-sulfate sodium salt -- DCA deoxycholic acid -- GLCA glycolithocholic acid -- TCDCA taurochenodeoxycholic acid -- CDCA chenodeoxycholic acid -- CA cholic acid -- TUDCA tauroursodeoxycholic acid dihydrate -- LCA lithocholic acid -- 3βUDCA 3β-ursodeoxycholic acid -- GCA glycocholic acid -- TLCA taurolithocholic acid -- UDCA ursodeoxycholic acid -- 3-DHCA 3-dehydrocholic acid -- α-MCA alpha-muricholic acid -- β-MCA beta-muricholic acid -- TCA taurocholic acid -- T-α-MCA tauro-alpha-muricholic acid -- GDCA glycodeoxycholic acid -- Ucp1 uncoupling protein 1 -- Pgc1α peroxisome proliferator-activated receptor γ (PPAR γ) coactivator-1α -- Prdm16 transcription factor PR domain containing 16 -- Cd137 tumor necrosis factor receptor superfamily member 9 -- Dio2 type 2 iodothyronine deiodinase 2 -- Cidea Cell death inducing DFFA like effector α -- Tbx1 T-box transcription factor 1 -- Elovl3 elongation of very long-chain fatty acids 3 -- Elovl6 elongation of very long-chain fatty acids 6; Lyophyllum decastes polysaccharide -- Anti-obesity -- Gut microbiota-bile acids-TGR5 axis -- Bacteroides intestinalis -- Lactobacillus johnsonii -- Energy expenditure; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119862
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Polysaccharides are known to confer protection against obesity via modulation of gut microbiota. To expand our knowledge of mushroom-derived prebiotics, we investigated the structural characteristics and anti-obesity effects of Lyophyllum decastes polysaccharides. Two heteroglycans were purified and characterized. The isolated polysaccharides effectively reduced obesity and the related disorders in the diet-induced obese (DIO) mice. An altered gut microbiota with enrichments of Bacteroides intestinalis and Lactobacillus johnsonii and an increase of secondary bile acids were detected in the polysaccharide-treated mice. Supplementation of B. intestinalis and L. johnsonii prevented the obesity and hyperlipidemia in DIO mice, demonstrating their causal linkage to the efficacy of polysaccharides. An enhancement of energy expenditure in the brown adipose tissues due to up-regulation of the secondary bile acids-activated TGR5 pathway was deduced to be one of the mechanisms underlying the effect of polysaccharides. These results confirmed Lyophyllum decastes -derived polysaccharides as new prebiotics for preventing and treating obesity.