Ultrasensitive electrochemical biosensor for microRNA-377 detection based on MXene-Au nanocomposite and G-quadruplex nano-amplification strategy. (1st October 2022)

• Record Type: Journal Article
• Authors: Wu, Qianqing; Li, Zhenhui; Liang, Qianwei; Ye, Rongkai; Guo, Shuzhou; Zeng, Xiaobing; Hu, Jianqiang; Li, Aiqing
• Is Part Of: Electrochimica acta. Volume 428(2022)
• Publication Date: 2022-10-01
• Subjects: MXene -- Au nanocomposites -- G-quadruplex -- MicroRNA-377 -- Electrochemical biosensor -- Nano-amplification; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2022.140945
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: The biosensor had wide linear range (10aM∼100pM) and low detection limit (1.35aM). The biosensor could realize accurate detection of miRNA-377 in human serum samples. The biosensor amplified electrochemical signal by embedding MB into G-quadruplexes. MXene-Au had high electrical conductivity and massive active sites for DNA capture. Abstract: MicroRNAs (miRNAs) are considered as potential biomarkers for early diagnosis and prognostic assessment of diabetic nephropathy. In this work, an electrochemical biosensor for ultrasensitive detection of miRNA-377 was constructed based on MXene-Au nanocomposites and G-quadruplex nano-amplification strategy. As a promising nanocarrier, taking advantage of leveraging synergistic effects between Au nanoparticles (AuNPs) and MXene nanosheet, MXene-Au nanocomposites exhibited excellent electronic conductivity and provided massive active sites for DNA capture probe immobilization by Au-S bonds. AuNPs modified with Guanine-rich sequence DNA detection probes were designed as signal amplification nano-labels. Specifically, by inducing the transition of Guanine-rich detection probes to G-quadruplex, the strong affinity interaction between methylene blue and G-quadruplex could not only reflect trace concentration information of miRNA-377, but also lead to the further enhancement of electrochemical signal (2.7-fold). As a result, this newly designed biosensor exhibited superior sensing performance with a wide linear range from 10 aM to 100 pM and the limit of detection was as low as 1.35 aM. Compared with biosensors based on other nanocomposites and reported miRNA-377 biosensors, the proposed sensing platform did not require any thermal cycling or reverse transcription process, meeting the miRNA sensing requirements of convenient, sensitive, specific and stable. Furthermore, the as-constructed biosensor also displayed good selectivity, which was applied to accurate detection of miRNA-377 in human serum samples with satisfactory sensitivity, suggesting the biosensor system has promising applications in biological researches and early clinical diagnosis for diabetic nephropathy. Graphical abstract: Image, graphical abstract .