SYNGR2 serves as a prognostic biomarker and correlates with immune infiltrates in esophageal squamous cell carcinoma. (1st August 2022)

• Record Type: Journal Article
• Authors: Li, Bin; Ren, Mei‐Yu; Chen, Yu‐Zhen; Meng, Yu‐Qi; Song, Tie‐Niu; Su, Zhi‐Peng; Yang, Bo
• Is Part Of: Journal of gene medicine. Volume 24:Number 8(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-08-01
• Subjects: esophageal squamous cell carcinoma -- immune infiltration -- prognosis -- synaptogyrin‐2; Genetic transformation -- Periodicals; Gene Transfer -- Periodicals; Gene Therapy -- Periodicals; 616.042
• DOI: 10.1002/jgm.3441
• Languages: English
• ISSNs: 1099-498X

Abstract

Abstract: Background: Synaptogyrin‐2 (SYNGR2) plays an important role in regulating membrane traffic in non‐neuronal cells. However, the role of SYNGR2 in esophageal squamous cell carcinoma (ESCC) remains unclear. Methods: All original data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and integrated via R 3.5.3. SYNGR2 expression was explored in the TCGA and GEO databases. The correlations between SYNGR2 and cancer immune characteristics were analyzed via the TIMER and TISIDB databases. Results: In general, SYNGR2 was predominantly overexpressed and had reference values in the diagnosis and prognostic estimation of ESCC. Upregulated SYNGR2 was associated with poorer overall survival, disease‐specific survival and T stage in ESCC. Mechanistically, we identified hub genes that included a total of 38 SYNGR2‐related genes, which were tightly associated with the protein polyubiquitination pathway in ESCC patients. SYNGR2 expression was negatively related to the infiltrating levels of T helper cells. SYNGR2 methylation was positively correlated with the expression of chemokines (CCL2 and CXCL12), chemokine receptors (CCR1 and CCR2), immunoinhibitors (CXCL12 and TNFRSF4) and immunostimulators (CSF1R and PDCD1LG2) in ESCC. Conclusion: SYNGR2 may be used as a biomarker for determining prognosis and immune infiltration in ESCC. Abstract : First, the sequencing data and clinical data of esophageal squamous cell carcinoma (ESCC) were downloaded from TCGA and GEO databases to further analyze the expression of SYNGR2 in cancer cells and adjacent tissues. Then the prognostic value of SYNGR2 in ESCC patients was evaluated, and the diagnostic value of SYNGR2 was evaluated via the receiver operating characteristic curve and nomogram. The genes closely related to SYNGR2 and the prognosis of ESCC were further screened and analyzed using enrichment analysis, protein–protein interaction analysis and correlation analysis. Finally, SYNGR2 and immune infiltrating cells in tumor microenvironment were analyzed using the TIMER and TISIDB databases.