Rapid and reagent-free bioassay using autobioluminescent yeasts to detect agonistic and antagonistic activities of bisphenols against rat androgen receptor and progesterone receptor. Issue 222 (September 2022)

• Record Type: Journal Article
• Authors: Huang, Yuan; Zhang, Wei; Zhang, Chengdong; Cui, Na; Xiao, Zhiming; Wang, Ruiguo; Su, Xiaoou
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 222(2022)
• Publication Date: 2022-09
• Subjects: Steroid hormone receptors -- Autobioluminescence -- Yeast -- In vitro -- Bisphenols; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2022.106151
• Languages: English
• ISSNs: 0960-0760

Abstract

Abstract: Bisphenol A (BPA) and its analogues have been classified as endocrine disruptors via binding to nuclear receptors. Two novel bioassays, BLYrARS and BLYrPRS, were developed for rapid detection of agonistic and antagonistic activities of BPA and five of its analogues binding rat androgen receptor (rAR) and rat progesterone receptor (rPR). The reporter bioassay was based on two autonomously bioluminescent strains of the yeast Saccharomyces cerevisiae, recombined with a bacterial luciferase reporter gene cassette (lux) that can produce autofluorescence, regulated by the corresponding hormone response element acting as the responsive promoter. The bioluminescent signal is autonomous and continuous without cell lysis or addition of exogenous reagents. The AR agonist R1881 could be detected at 4 h with a half-maximal effective concentration (EC50 ) of ~9.4 nM. The PR agonist progesterone could be determined at 4 h with an EC50 of ~2.74 nM. None of the sixteen bisphenols presented agonistic activities against rAR and rPR. However, thirteen BPs were rAR antagonists and eleven BPs acted as rPR antagonists with different potency. The BLYrARS and BLYrPRS bioassay characterized by automated signal acquisition without additional manipulations or cost can be applied for simple and rapid detection of agonistic and antagonistic activities of BPs and other compounds acting as agonists or antagonists of rAR and rPR. Based on data derived by use of this bioassay endocrine-disrupting activities of some BPA analogues are more potent than BPA. Graphical Abstract: ga1 Highlights: Two novel bioassays using autobioluminescent S. cerevisiae yeasts, BLYrARS and BLYrPRS, were developed. Bioassays can be applied for easy handling, robustly and cost-effectively detecting activities of BPs against rAR or rPR. Thirteen BPs presented rAR antagonistic activities and eleven BPs presented rPR antagonistic activities. Endocrine-disrupting activities of some BPA analogues are more potent than BPA.