Combined immunological and histochemical analysis of skin and lymph node lesions in histiocytosis X. Issue 3 (March 1982)

• Record Type: Journal Article
• Authors: Thomas, J A; Janossy, G; Chilosi, M; Pritchard, J; Pincott, J R
• Is Part Of: Journal of clinical pathology. Volume 35:Issue 3(1982)
• Page Start: 327
• Page End: 337
• Publication Date: 1982-03
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.35.3.327
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : The immunological phenotype of the cells involved in skin and lymph node lesions from two cases of histiocytosis X (H-X) were analysed by immunofluorescence techniques using combinations of heterologous and monoclonal antisera to Ia-like antigen and human cortical thymocyte (HTA-1) determinant. These cells were also characterised by a new technique using simultaneous immunofluorescence and enzyme histochemistry for acid phosphatase (ACPase). The major cell type in the lesions was found to express the same Ia+, HTA-1+ phenotype as normal epidermal Langerhans' cells (LC) and was unreactive for ACPase. Additional cell types included Ia-, HTA-1- multinucleate giant cells and residual lymphoid populations. These findings endorse previous concepts that H-X is a proliferation of abnormal LC and emphasise the heterogeneous nature of the cells involved in the disease.