Discovering the effect of solvents on poly(2-aminoethyl methacrylate) grafting onto chitosan for an in vitro skin model. (1st November 2022)

• Record Type: Journal Article
• Authors: González-Torres, Maykel; Vargas-Muñoz, Susana; Leyva-Gómez, Gerardo; Méndez-Padilla, Ma. Guadalupe; Cortés, Hernán; Nuñez-Rojas, Edgar; González-Mendoza, Oswaldo; Pérez-Díaz, Mario Alberto; Ruvalcaba-Paredes, Erika Karina; Lima, Enrique; Brena, Ana María; Rodríguez-Talavera, Rogelio; Pineda, Carlos
• Is Part Of: Carbohydrate polymers. Volume 295(2022)
• Publication Date: 2022-11-01
• Subjects: CS Chitosan -- PS Polysaccharide -- AEMA 2-Aminoethyl methacrylate -- PAEMA Poly(2-aminoethyl methacrylate) -- CS-g-PAEMA Poly(2-aminoethyl methacrylate) grafted onto CS -- PU Polyurethane -- HDI Hexamethylene diisocyanate -- IF Immunofluorescence -- CLS Cellulose -- CTN Chitin -- -NH2 Amino groups -- -OH Hydroxyl groups -- DMA (N, N-dimethylamine) ethyl methacrylate -- CECTS N-carboxyethyl chitosan -- GRIR Gamma radiation-induced reactions -- THF Tetrahydrofuran -- PP Polypropylene -- DMSO Dimethyl sulfoxide -- St Styrene -- ETOH Ethanol -- HEX Hexane -- EA Ethyl acetate -- CL Chloroform -- AE Acetone -- AA Acetic acid -- CSM1S1 or S1 CS-g-PAEMA prepared in ETOH -- CSM1S2 or S2 CS-g-PAEMA prepared in HEX -- CSM1S3 or S3 CS-g-PAEMA prepared in EA -- CSM1S4 or S4 CS-g-PAEMA prepared in CL -- CSM1S5 or S5 CS-g-PAEMA prepared in AE -- CSM1S6 or S6 CS-g-PAEMA prepared in AA -- FTIR Fourier-transform infrared -- TGA Thermal gravimetric analysis -- DSC Differential scanning calorimetry -- XPS X-ray photoelectron spectra -- HDFs Human dermal fibroblasts -- MTT 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- FSP Anti-fibroblast surface protein -- GaAsP Gallium arsenide phosphide -- OPLS LigParGen-Optimized Potentials for Liquid Simulations -- RDF Radial distribution functions -- SFE Solvation free energy -- MD Molecular dynamics -- NPT Commonly used ensemble during MD simulation of molecules -- MDH Mitochondrial dehydrogenase; Chitosan -- Poly(2-aminoethyl methacrylate) -- Graft copolymerization -- Structural characterization -- γ-Radiation -- Grafting -- Effect of solvent -- Grafting degree; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119864
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The design of controlled grafting copolymers is critical in synthesizing effective artificial cellular matrices because of their regulatory role in cellular behavior. However, it is unclear whether poly(2-aminoethyl methacrylate) grafted onto chitosan generated by gamma-radiation-induced graft polymerization in different solvents can influence the physicochemical properties and biotech applicability of the copolymer. This work aims to demonstrate for the first time the effect of various solvents on the synthesis, properties, and biological performance of grafted chitosan using the simultaneous irradiation method. The results proved that the solvent is one of the critical factors affecting the properties of the modified polysaccharide. The degree of grafting showed a solvent-dependent profile. Hexane presented utmost importance concerning the degree of grafting. Ethyl acetate showed the best results in grafting extent and human dermal fibroblast growth. These findings indicate that proper solvent selection determines the possible copolymer use for in vitro engineered skin substitute models. Graphical abstract: Unlabelled Image Highlights: The effect of solvent on CS-g-PAEMA synthesis and performance was investigated. The degree of grafting and grafting extent showed a solvent-dependent profile. The choice of the solvent also influenced the in vitro HDFs grown in the copolymer. Hexane presented utmost importance concerning the degree of grafting dependence. Ethyl acetate showed maximum graft extent, cell growth, and proliferation results.