A novel lipid droplets-specific fluorescent bio-probe with large Stokes shift for the marking of living cells and zebrafish. (27th August 2022)

• Record Type: Journal Article
• Authors: Xuan, Yunpeng; Zhang, Zhi-Hao; Cui, Wei-Long; Chen, Xiao-Qian; Qu, Jianbo; Wang, Jian-Yong
• Is Part Of: Tetrahedron. Volume 121(2022)
• Publication Date: 2022-08-27
• Subjects: Fluorescent probe -- Lipid droplets -- Large Stokes shift -- Cell labeling; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2022.132915
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: In eukaryotes, lipid droplets (LDs) composed of phospholipid monolayers and neutral lipids are crucial spherical subcellular organelles with diameters ranging from 40 nm to 100 μm. LDs play a pivotal role in various physiological activities and are closely associated with many diseases. Therefore, it is essential to develop a fluorescent probe with excellent photostability and large Stokes shift (∼100 nm) for tracking LDs. Herein, a novel lipid droplet-specific fluorescent bioprobe based on coumarin-substituted p-nitrophenyl ethylene was designed and constructed, which exhibited the desirable effects of excellent photostability, favorable resistance to ionic interference, low cytotoxicity and LDs specificity. As a proof of principle, we labeled and imaged LDs of HeLa cells by Cou-LDs successfully. Graphical abstract: In eukaryotes, lipid droplets (LDs) composed of phospholipid monolayers and neutral lipids are crucial spherical subcellular organelles with diameters ranging from 40 nm to 100 μm. LDs play a pivotal role in various physiological activities and are closely associated with many diseases. Therefore, it is essential to develop a fluorescent probe with excellent photostability and large Stokes shift (∼100 nm) for tracking LDs. Herein, a novel lipid droplet-specific fluorescent bioprobe based on coumarin-substituted p-nitrophenyl ethylene was designed and constructed, which exhibited the desirable effects of excellent photostability, favorable resistance to ionic interference, low cytotoxicity and LDs specificity. As a proof of principle, we labeled and imaged LDs of HeLa cells by Cou-LDs successfully. Image 1